Figure 4

Schematic representation of the « single color/resistance » PRIG vectors. pPRICp aHA, pPRIYp aHA and pPRIChp aHA and pPRIPu vectors are pPRIG derivatives in which the eGFP sequence from the pPRIG has been replaced by eCFP*, eYFP, mCherry or Puro-R coding sequence, respectively. eCFP* is a derivative of eCFP bearing the H148D mutation which enhances its brightness. pPRICp aHA, pPRIYp aHA and pPRIChp aHA contain an in-frame 3' HA sequence (frame « a ») with respect to the MCS. They also contain the two PacI sites flanking the IRES-XFP cassette (shown as vertical bars in pPRIYp aHA and pPRIChp aHA). By contrast, pPRIPu is devoid of both HA sequence and PacI sites. For each vector, the unique sites of the MCS are indicated above the MCS. Unique sites of the pPRIYp aHA MCS are not listed since they are the same ones as in the immediately above vector.