Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Table 2 Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with siRNA expression vectors

Clone Name	siRNA^a	LF^b	Relative composition of monosaccharide			Fucose(-)%^d
			Fucose^c	GlcNAc	Mannose
32-05-12	-	-	0.97	4.00	2.67	3
GMDb2	GMD	-	0.22	4.00	2.52	78
GMDb5	GMD	-	0.21	4.00	2.53	79
GMDb2-F3	GMD·FUT8	-	0.02	4.00	2.70	98
GMDb2-F5	GMD·FUT8	-	0.03	4.00	2.71	97
GMDb5-F2	GMD·FUT8	-	0.05	4.00	2.77	95
GMDb5-F4	GMD·FUT8	-	0.09	4.00	2.70	91
32-05-12	-	+	0.94	4.00	2.56	6
GMDb2	GMD	+	0.94	4.00	2.56	6
GMDb5	GMD	+	0.92	4.00	2.60	8
GMDb2-F3	GMD·FUT8	+	0.19	4.00	2.52	81
GMDb2-F5	GMD·FUT8	+	0.21	4.00	2.54	79
GMDb5-F2	GMD·FUT8	+	0.19	4.00	2.55	81
GMDb5-F4	GMD·FUT8	+	0.33	4.00	2.68	67

^aGMD, introduction of GMD siRNA expression vector; GMD·FUT8, introduction of FUT8 siRNA expression vector to GMD siRNA-introduced cells.
^bLF, addition of 100 μM L-fucose into the culture medium.
^cMolar ratios calculated vs. 4 GlcNAc.
^dTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-c) × 100.

ISSN: 1472-6750