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Table 2 Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with siRNA expression vectors

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Clone Name siRNAa LFb Relative composition of monosaccharide Fucose(-)%d
    Fucosec GlcNAc Mannose  
32-05-12 - - 0.97 4.00 2.67 3
GMDb2 GMD - 0.22 4.00 2.52 78
GMDb5 GMD - 0.21 4.00 2.53 79
GMDb2-F3 GMD·FUT8 - 0.02 4.00 2.70 98
GMDb2-F5 GMD·FUT8 - 0.03 4.00 2.71 97
GMDb5-F2 GMD·FUT8 - 0.05 4.00 2.77 95
GMDb5-F4 GMD·FUT8 - 0.09 4.00 2.70 91
32-05-12 - + 0.94 4.00 2.56 6
GMDb2 GMD + 0.94 4.00 2.56 6
GMDb5 GMD + 0.92 4.00 2.60 8
GMDb2-F3 GMD·FUT8 + 0.19 4.00 2.52 81
GMDb2-F5 GMD·FUT8 + 0.21 4.00 2.54 79
GMDb5-F2 GMD·FUT8 + 0.19 4.00 2.55 81
GMDb5-F4 GMD·FUT8 + 0.33 4.00 2.68 67
  1. aGMD, introduction of GMD siRNA expression vector; GMD·FUT8, introduction of FUT8 siRNA expression vector to GMD siRNA-introduced cells.
  2. bLF, addition of 100 μM L-fucose into the culture medium.
  3. cMolar ratios calculated vs. 4 GlcNAc.
  4. dTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-c) × 100.