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Figure 5 | BMC Biotechnology

Figure 5

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Figure 5

Structure of siRNA expression plasmids. The GMD siRNA expression plasmid consisted of a puromycin resistance gene and a short hairpin siRNA expression cassette controlled by the human U6 promoter (A). FUT8 siRNA expression plasmids consisted of a puromycin or hygromycin resistance gene and a short hairpin siRNA expression cassette controlled by the human tRNAval promoter (B or C). The siRNA tandem expression plasmid consisted of a hygromycin resistance gene and two short hairpin siRNA expression cassettes targeting FUT8 and GMD (D). The transcribed shRNAs and tRNA-shRNA fusion product were processed into siRNAs by Dicer.

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BMC Biotechnology

ISSN: 1472-6750

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