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Figure 4 | BMC Biotechnology

Figure 4

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Figure 4

Biological activity of antibodies from clones transformed with the FUT8 and GMD siRNA tandem expression vector. Lysis of antigen-expressing cells targeted by human PBMCs at a target:effector ratio of 1:20 in the presence of different antibody concentrations was quantified by detecting lactate dehydrogenase activity (A). The antigen-binding activity of the antibody was measured by ELISA (B). Antibody purified from the serum-free fed-batch cultures of cells transformed by the FUT8 and GMD tandem siRNA expression vector, FG1 (open circles), FG16 (open squares), and the parental cell line 32-05-12 (filled circles) are shown. Cytotoxicity (%) and absorbance are indicated as the mean values ± SD of triplicates.

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BMC Biotechnology

ISSN: 1472-6750

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