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Figure 3 | BMC Biotechnology

Figure 3

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Figure 3

Serum-free fed-batch culture of clones transformed by the FUT8 and GMD tandem siRNA expression vector. Serum-free fed-batch culture using a 1L spinner bioreactor was carried out using cells transformed by the FUT8 and GMD tandem siRNA expression vector (FG1 (open circles) and FG16 (filled circles)). The parental cell line 32-05-12 (filled squares) was cultured as a control. Viable cell density (A, solid lines), antibody concentration in the culture supernatant (A, dotted lines), and cell viability (B) were analyzed in the fed-batch culture. The oligosaccharide structures of the final products from 32-05-12 (C), FG1 (D), and FG16 (E) were analyzed using MALDI-TOF MS. The relative composition of each peak is shown as the relative amount to the total amount of oligosaccharide detected.

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BMC Biotechnology

ISSN: 1472-6750

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