Figure 1

Polyclonal phage ELISA. Phage pools obtained from panning the new SPSL1 with the three tested toxins and panning the previously established SP with SEB and ricin were tested for binding specificity using ELISA. After three rounds of panning SPSL1, SEB binder phages were enriched approximately three-fold, while no enrichment was observed from panning SP (A). Ricin binder phages were enriched over ten-fold after second and third panning of SPSL1 and no enrichment was observed in panning SP (B). BoNT/A complex toxoid binders were enriched approximately twelve-fold after the first round and there was no significant enrichment after the second round. The same amount of the phage from each sample was used for the assay. Data were obtained from two independent experiments.