Figure 2From: Revealing biases inherent in recombination protocolsTemplates-pairs used for the recombination of mRFP (black) and DsRed (grey). Parental sequences or parental background are either mRFP WT or DsRed WT genes. Digested GFP pProTeT plasmids were used as cloning vectors for inserts to ensure that only fully cut plasmids were ligated with chimeric inserts.Back to article page