Table 1 Plasmid templates used in qPCR
Plasmid Name | Parental Vector | Amplicon Size | Insert Origin | 5'-End Sequence of the Insert | 3'-End Sequence of the Insert |
|---|---|---|---|---|---|
pMcG | pUC18 | 121 | Human myc gene | TCAAGAGGTGCCACGTCTCC | CTGATCTGTCTCAGGACTCT |
pTBP | pCRII-TOPO | 228 | Human TBP cDNA | AGCAAGAAAATATGCTAGAG | TTTCCAGAAACAAAAATAAG |
pGCL | pBluescript | 529 | T5 DNA polymerase | CCAGAACCATACAGAATACC | CCAGAACCATACAGAATACC |