Figure 5From: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applicationsNormalized binding curves of EG (at 11.15 μM) and SG (at 11.42 μM) with various oligonucleotides in 100 mM Tris, pH 8.0 at room temperature : 1) EG + ds22-mer (dark solid line); 2) EG + ds10-mer (dark dashed line); 3) EG + ss24-mer (dark dotted line); 4) SG + ds22-mer (gray solid line); 5) SG + ds10-mer (gray dashed line); and 6) SG + ss24-mer (gray dotted line).Back to article page