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Figure 5 | BMC Biotechnology

Figure 5

From: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications

Figure 5

Normalized binding curves of EG (at 11.15 μM) and SG (at 11.42 μM) with various oligonucleotides in 100 mM Tris, pH 8.0 at room temperature : 1) EG + ds22-mer (dark solid line); 2) EG + ds10-mer (dark dashed line); 3) EG + ss24-mer (dark dotted line); 4) SG + ds22-mer (gray solid line); 5) SG + ds10-mer (gray dashed line); and 6) SG + ss24-mer (gray dotted line).

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