Figure 1

Absorption spectra of EG in the presence of various amounts of λDNA: 0 ng/μL (dotted line); 5 ng/μL (line 1), 10 ng/μL (line 2), 25 ng/μL (line 3) and 100 ng/μL (line 4). All spectra were measured at room temperature in 100 mM Tris buffer, pH 8.0 with an EG concentration of 11.15 μM. The arrows indicate trends of absorbance change in response to the increase of DNA amount. All absorbance points are relative to the maximum of line 4.