Figure 3

Visualisation of LucF+ in mice after bicistronic vector electrotransfer in tibialis anterior muscle of C57BL/6J mice and intraperitoneal injection of D-luciferin. A. Imaging of bioluminescent signal detected with a CCD camera. LucF+ activity was measured in mice electrotransferred with bicistronic luciferase vectors (expression of LucF+ is under the translational control of EMCV, FGF-2 or FGF-1A IRES). Images are shown in pseudocolors. Time of exposure for each image and a pseudocolor scale are represented. *: non electrotransferred muscle. B. Mean of bioluminescent signal quantification. Signal quantification was expressed in mean grey level/sec/ROI* (± sem). For each time point, n = 8 for EMCV, n = 10 for FGF-2 and n = 8 for FGF-1A. * Region Of Interest, corresponds to the bioluminescent area of the electrotransferred muscle.