Table 2 Bacterial strains and plasmids used in this study
From: An integrative expression vector for Actinosynnema pretiosum
Strain or plasmid | Relevant characteristic | Purpose | Reference/Source |
|---|---|---|---|
Amycolatopsis mediterranei | Rifamycin producer | Amplification of rifH | ATCC |
S. coelicolor A(3)2 | IS117 transposable elements | Amplification of IS117 | ATCC |
A. pretiosum 31565 | AHBA biosynthesis gene cluster | Amplification of asm19 RBS, conjugation | ATCC |
E. coli | Â | Â | Â |
   ET12567/pUZ8002 | Methylation-deficient host with non-transmissible helper plasmid | Conjugation host | [25] |
Plasmids | Â | Â | Â |
   pKS1 | aprR, ori | Vector backbone | This study |
   pXE3 | xylE | Amplification of xylE | [25] |
   pAP40 | ermE*p, IS117, aac(3)IV | Assessment of IS117 | This study |
   pAP41 | Derived from pAP40, ermE RBS, xylE | Assessment of RBS | This study |
   pAP42 | Derived from pAP40, E. coli RBS, xylE | Assessment of RBS | This study |
   pAP43 | Derived from pAP40, putative asm19 RBS, xylE | Assessment of RBS | This study |
   pAP44 | Derived from pAP42, E. coli RBS, rifH | Construction of translational reporter | This study |
   pAP47 | Derived from pAP44, E. coli RBS, rifH-xylE fusion | Assessment of translational reporter | This study |
   pAP50 | Derived from pAP44, E. coli RBS, rifH-xylE operon construct | Assessment of translational reporter | This study |