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Table 2 Bacterial strains and plasmids used in this study

From: An integrative expression vector for Actinosynnema pretiosum

Strain or plasmid Relevant characteristic Purpose Reference/Source
Amycolatopsis mediterranei Rifamycin producer Amplification of rifH ATCC
S. coelicolor A(3)2 IS117 transposable elements Amplification of IS117 ATCC
A. pretiosum 31565 AHBA biosynthesis gene cluster Amplification of asm19 RBS, conjugation ATCC
E. coli    
   ET12567/pUZ8002 Methylation-deficient host with non-transmissible helper plasmid Conjugation host [25]
Plasmids    
   pKS1 aprR, ori Vector backbone This study
   pXE3 xylE Amplification of xylE [25]
   pAP40 ermE*p, IS117, aac(3)IV Assessment of IS117 This study
   pAP41 Derived from pAP40, ermE RBS, xylE Assessment of RBS This study
   pAP42 Derived from pAP40, E. coli RBS, xylE Assessment of RBS This study
   pAP43 Derived from pAP40, putative asm19 RBS, xylE Assessment of RBS This study
   pAP44 Derived from pAP42, E. coli RBS, rifH Construction of translational reporter This study
   pAP47 Derived from pAP44, E. coli RBS, rifH-xylE fusion Assessment of translational reporter This study
   pAP50 Derived from pAP44, E. coli RBS, rifH-xylE operon construct Assessment of translational reporter This study