Construction of A. pretiosum vectors. Maps of plasmids with relevant features and single restriction sites are shown. Site directed mutagenesis of IS117 for a functional attM is indicated in pAP3. pAP41, pAP42 and pAP43 consist of xylE with RBS sequences from S. erythraea, E. coli and A. pretiosum genes, respectively, although only the E. coli RBS sequence is indicated. The aac(3)IV gene encoding apramycin resistance is represented by aprR.