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Figure 4 | BMC Biotechnology

Figure 4

From: Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains

Figure 4

Purification of VH domain antibodies. A) Total lysates and soluble fractions obtained after periplasmic extraction of bacteria expressing the soluble VHs C36 and C47, as well as the insoluble VH DP47. B) Heat-treatment and affinity purification of C47. The supernatant obtained after periplasmic extraction was either heated 20 min at 70°C or loaded onto a cobalt-activated column for IMAC.

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BMC Biotechnology

ISSN: 1472-6750

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