Table 1 Mortality induced by different volumes of injection. The disruptive effect of injection volume was estimated by testing three different volumes (46, 23 and 5 nl) of cathepsin-L dsRNA, bacterial LacZ control dsRNA, or water. For each tested volume, 20 L3 were injected and the mortality within the two days following the injection was measured and expressed as a percentage of injected aphids.
From: Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum
Injected volume | Injected material | Mortality (%) |
|---|---|---|
46 nl | ds1-Ap-cath-L | 45 |
ds-LacZ | 38 | |
H2O | 29 | |
23 nl | ds-LacZ | 11 |
H2O | 13 | |
5 nl | ds-LacZ | 16 |
H2O | 14 |