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Table 2 IFN-γ production and protection in swine immunized with chimeric virus BVP1

From: Induction of protective immunity in swine by recombinant bamboo mosaic virus expressing foot-and-mouth disease virus epitopes

   IFN-γ (fold change)a  
Antigens Swine No. rVP1 PHA Clinical symptomsb
10 mg BVP1 858 3.8 2.4 No
  859 4.1 2.8 No
  860 3.4 2.2 No
5 mg BVP1 861 3.1 2.9 No
  862 3.8 2.1 No
  863 2.4 2.6 No
5 mg BaMV-S 864 1.0 2.5 Yes
  865 1.1 2.2 Yes
PBS 866 1.0 2.6 Yes
  867 1.0 2.4 Yes
  1. a PBMCs isolated from swine 10 weeks after priming were stimulated with 5 μg/ml of rVP1 or 1 μg/ml of PHA for 6 h at 37°C. Total cellular RNA was then extracted for the assay of IFN-γ production by real-time PCR. The fold change was determined by dividing the quantity of specific IFN-γ mRNA from stimulated cells by the quantity of IFN-γ mRNA from the unstimulated cells.
  2. b Swine of all groups were challenged with FMDV (O/Taiwan/97) and monitored daily for clinical symptoms of FMD for 14 days as described in Methods.