Effective in vivo silencing of luciferase in subcutaneous and intracranial tumor models. (A) Mean intensity luciferase expression indicates Dox induced luciferase knockdown in subcutaneous tumor xenograft at day 3 and 5 post-injection. Eight animals are represented for each treatment condition. (B) Dox treatment or luciferase knockdown does not reduce tumor growth of SVT2 cells in SCID mice. (C) Mean intensity luciferase expression demonstrates Dox-induced luciferase knockdown relative to controls at days 3 (9.5 fold), 5 (13.8 fold) and 9 (11 fold) post-injection. The luminescence values for each time point have been normalized to the respective intensity value at day 0. Five animals are represented for each treatment condition. Representative BLI images are shown in additional file 7.