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Table 1 RNA metrics associated with RIN value and RNA degradation method. Average values and standard deviations are reported for metrics associated with samples in each RIN class (n = 3).

From: Characterization of the effect of sample quality on high density oligonucleotide microarray data using progressively degraded rat liver RNA

Handling condition RIN class RIN 28s/18s rRNA cRNA yield cRNA length 3'/5' GAPDH 3'/5' β-actin
F/T 9.5 9.5 ± 0.1 1.43 ± 0.15 86 ± 8 1923 ± 157 1.17 ± 0.06 1.70 ± 0.35
  8 8.1 ± 0.2 1.00 ± 0.00a 63 ± 4b 1398 ± 116a 1.77 ± 0.21 1.93 ± 0.06
  7 7.0 ± 0.1 0.70 ± 0.10a 44 ± 12a 1136 ± 207a 3.05 ± 0.95b 3.62 ± 1.18b
  6 6.2 ± 0.1 0.50 ± 0.10a 38 ± 3a 878 ± 62a 5.50 ± 0.8a 5.27 ± 0.86a
37°C 9.5 9.5 ± 0.2 1.35 ± 0.13 74 ± 16 2294 ± 236 1.01 ± 0.02 1.34 ± 0.02
  9 9.1 ± 0.1 1.33 ± 0.06 83 ± 7 2110 ± 322 1.24 ± 0.20 1.49 ± 0.26
  8 8.2 ± 0.1 1.33 ± 0.06 61 ± 19 1834 ± 293 1.29 ± 0.18 1.60 ± 0.04
  7 7.0 ± 0.2 1.07 ± 0.15b 42 ± 6 1210 ± 158a 2.00 ± 0.21a 2.12 ± 0.20a
  6 6.1 ± 0.5 0.90 ± 0.10a 39 ± 18a 1244 ± 223a 1.96 ± 0.41a 2.29 ± 0.40a
  1. aP < 0.01 compared to RIN 9.5 set
  2. bP < 0.05 compared to RIN 9.5 set