Handling condition | RIN class | RIN | 28s/18s rRNA | cRNA yield | cRNA length | 3'/5' GAPDH | 3'/5' β-actin |
---|---|---|---|---|---|---|---|
F/T | 9.5 | 9.5 ± 0.1 | 1.43 ± 0.15 | 86 ± 8 | 1923 ± 157 | 1.17 ± 0.06 | 1.70 ± 0.35 |
8 | 8.1 ± 0.2 | 1.00 ± 0.00a | 63 ± 4b | 1398 ± 116a | 1.77 ± 0.21 | 1.93 ± 0.06 | |
7 | 7.0 ± 0.1 | 0.70 ± 0.10a | 44 ± 12a | 1136 ± 207a | 3.05 ± 0.95b | 3.62 ± 1.18b | |
6 | 6.2 ± 0.1 | 0.50 ± 0.10a | 38 ± 3a | 878 ± 62a | 5.50 ± 0.8a | 5.27 ± 0.86a | |
37°C | 9.5 | 9.5 ± 0.2 | 1.35 ± 0.13 | 74 ± 16 | 2294 ± 236 | 1.01 ± 0.02 | 1.34 ± 0.02 |
9 | 9.1 ± 0.1 | 1.33 ± 0.06 | 83 ± 7 | 2110 ± 322 | 1.24 ± 0.20 | 1.49 ± 0.26 | |
8 | 8.2 ± 0.1 | 1.33 ± 0.06 | 61 ± 19 | 1834 ± 293 | 1.29 ± 0.18 | 1.60 ± 0.04 | |
7 | 7.0 ± 0.2 | 1.07 ± 0.15b | 42 ± 6 | 1210 ± 158a | 2.00 ± 0.21a | 2.12 ± 0.20a | |
6 | 6.1 ± 0.5 | 0.90 ± 0.10a | 39 ± 18a | 1244 ± 223a | 1.96 ± 0.41a | 2.29 ± 0.40a |