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Table 1 Primers used for the amplification of the HPV-11 L1 and L1 NLS- ORFs

From: Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum

Primer Pair

Construct

Primers

1

pHPL51 (502)

Forward 5'- GGAATTCCGGAGGAATTTTTTACAGATGTGGCGGC -3'

  

Reverse 5'- GACGCCGTCGACTCCGGAACTGACACACATATATTAG -3'

2

pHPS51 (ΔC481)

Forward 5'- GGAATTCCGGAGGAATTTTTTACAGATGTGGCGGC -3'

  

Reverse 5'- ACGCCGTCGACTCCGGATTATATACCTGTTACGAGCAGC -3'

3

pART27 L1

Forward 5'- ATGTGGCGGCCTAGCGAC -3'

  

Reverse 5'- TTACTTTTTGGTTTTGGTACGTTTTCG-3'

4

pART27 L1 NLS-

Forward 5'- ATGTGGCGGCCTAGCGAC -3'

  

Reverse 5'- TTATATACCTGTACGAGCAGACG -3'

5

HPV-11 L1 NLS- internal

Forward 5'- TGATACAGGCTTTGGTGC -3'

  

Reverse 5'- TGGCTCATTGGTGTCTTC -3'

  1. GAATTC: EcoRI; TCCGGA: MroI, GTCGAC: SalI, ATG = start codon, TAA = stop codon
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BMC Biotechnology

ISSN: 1472-6750

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