Western blot analysis of plant extracts. (A) Western blot analysis of TCA-precipitated A. thaliana protein extract detected with H6:E51. HPV-11 L1 NLS- protein is present in both protein extracts from primary bolts and complete plants. M = MW marker; B = bolts; Pl = plants. (B) Western blot analysis of TCA precipitated N. tabacum protein extract detected with H6:I2. The HPV-11 L1 NLS- protein is present in all generations of the N. tabacum transgenic lines and is ~36 kDa in size. (-) represents the TCA precipitated non-transgenic control. The positive controls (+) and (+)* are insect cell-derived CsCl-purified HPV-11 L1 (NLS+) and HPV-11 L1 NLS- VLPs, respectively. (C) Western blot analysis of non-concentrated, non-purified HPV-11 L1 NLS- VLPs in insect cell lysate detected with H6:C6 (IC). Non-infected insect cell lysate was used as negative control (-) in C.