Genomic DNA analysis of transgenic plants. (A) Analysis of individual transgenic A. thaliana lines of the T6 generation (A1-4 represent 4 plants of line A; B1-4 plants from line B etc.), indicating the presence of the HPV-11 L1 NLS- gene. (B) Screening of genomic DNA extracted from transgenic N. tabacum line X1 (T1 generation) plants for the presence of the HPV-11 L1 NLS- gene. The majority of the individual plants are positive for the HPV-11 L1 NLS- gene, as is shown by a 1.5 kb PCR amplification product. Genomic DNA extracted from respective non-transgenic plants and HPV-11 L1 NLS- plasmid DNA represent the negative and positive controls respectively. (C) RT-PCR analysis of PCR positive transgenic A. thaliana plants from the T1, T2, T3 and T6 generations and (D) of individual T1, T2 and T3 generation N. tabacum plants from lines X1, Xi, and Xk. (for example: Xi 2.5 represents line Xi, T2 generation, plant number 5). Individual plants from the respective lines express the L1 NLS- gene as shown by the 303 bp amplification product. Total RNA extracted from respective non-transgenic plants represent the negative control.