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Table 1 Primers and probe used in this study

From: Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilacticiUVA1

Primer/probe Target/specificity Sequence (5'-3') Product size (bp) Reference
Bak11w 16S rDNA AGT TTG ATC MTG GCT CAG   [35]
Bak4   AGG AGG TGA TCC ARC CGC A   [36]
P1 pedA-pedB AAA ATA TCT AAC TAA TAC TTG   
P2   TAA AAA GAT ATT TGA CCA AAA 711 [23]
pedopF pedB-pedD GGG CGA GTT TAA CAT GCT AGA   
pedopR   TGA TTA TGA ATT AAC CGT GCA 2862 This study
pedseqA Sequencing of the pediocin operon CTT GCT CGA TAA TGG TAA   
pedseqB   CTA TCA GGT AAC TGA AAA   
pedseqC   CAC GCT TTT CTG ATG CAA   
pedseqD   TTC TTG ACC CCA TTA GAA   This study
pedseqL Upstream of operon AGC AAT TAC AGT CAA CCA TAA CCA T   
pedseqH   ACA GAG CAG GAA TGT TTG CCA CAA G 1009 This study
pedseq M Downstream of operon ATT GTC TCC GGC ACA ATG TT   
pedseqN   CAA AGT GGG GAA ACT CGA AA 1417 This study
pedA2RTF Real-time PCR GGC CAA TAT CAT TGG TGG TA   
pedA2RTR   ATT GAT TAT GCA AGT GGT AGC C   
TqM-pedA   FAM-ACT TGT GGC AAA CAT TCC TGC TCT GTT GA-TAMRA 100 This study