Localization of the pedA gene on the plasmid by curing and Southern blotting. (a) Agarose gel electrophoresis of plasmid DNA. UVA1: P. acidilactici UVA1. UL5: P. acidilactici UL5. bac-: cured derivative of UVA1. M. Supercoiled DNA ladder in kb. ch: chromosomal DNA band. (b) Southern blot DNA hybridization of the DIG labeled pedA-probe with plasmidic DNA from P. acidilactici bac-, UVA1 and UL5. (c) and (d) Agar-well diffusion assay with CFS of cultures of P. acidilactici UVA1 (c) and its cured derivative P. acidilactici bac- (d).