Table 1 Experimental design used in RSM and ANN studies by using four independent variables showing observed values of percentage yield of wax esters synthesis
Temperature (°C) | Time (h) | Molar ratio (mmol) | Amount of enzyme (g) | Actual yield (%) mean ± SD |
|---|---|---|---|---|
40 | 2.5 | 2 | 0.1 | 30.2 ± 0.17 |
60 | 2.5 | 2 | 0.1 | 32.9 ± 0.52 |
60 | 7.5 | 2 | 0.1 | 75.5 ± 0.82 |
40 | 2.5 | 4 | 0.1 | 41.8 ± 0.46 |
40 | 7.5 | 4 | 0.1 | 75.2 ± 1.12 |
60 | 7.5 | 4 | 0.1 | 72.9 ± 0.57 |
60 | 2.5 | 2 | 0.2 | 55.6 ± 0.23 |
40 | 7.5 | 2 | 0.2 | 80.8 ± 0.37 |
60 | 7.5 | 2 | 0.2 | 78.7 ± 0.19 |
60 | 2.5 | 4 | 0.2 | 60.6 ± 0.41 |
40 | 7.5 | 4 | 0.2 | 72.4 ± 0.6 |
60 | 7.5 | 4 | 0.2 | 68.6 ± 0.36 |
30 | 5 | 3 | 0.15 | 48.5 ± 0.18 |
70 | 5 | 3 | 0.15 | 48 ± 0.3 |
50 | 0 | 3 | 0.15 | 20 ± 0.15 |
50 | 10 | 3 | 0.15 | 80 ± 0.77 |
50 | 5 | 1 | 0.15 | 70.2 ± 0.66 |
50 | 5 | 5 | 0.15 | 72.5 ± 0.85 |
50 | 5 | 3 | 0.05 | 70.1 ± 1.21 |
50 | 5 | 3 | 0.25 | 82 ± 0.49 |
50 | 5 | 3 | 0.15 | 81.6 ± 0.28 |
50 | 5 | 3 | 0.15 | 82.0 ± 0.51 |
50 | 5 | 3 | 0.15 | 82.4 ± 0.43 |
50 | 5 | 3 | 0.15 | 80.0 ± 0.45 |
50 | 5 | 3 | 0.15 | 82.5 ± 0.78 |
50 | 5 | 3 | 0.15 | 82.7 ± 0.39 |
40 | 7.5 | 2 | 0.1 | 75.2 ± 1.11 |
60 | 2.5 | 4 | 0.1 | 45.5 ± 0.3 |
40 | 2.5 | 2 | 0.2 | 46.2 ± 0.26 |
40 | 2.5 | 4 | 0.2 | 55.4 ± 1.44 |