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Figure 5 | BMC Biotechnology

Figure 5

From: Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac

Figure 5

Expression levels induced by constitutive piggyBac vectors. The genetic structures of the different constitutive piggyBac vectors assayed in this experiment, with the differences in orientation of the heterologous genes indicated by A and B, are shown above the plots in Columns A, B, and C. Column A shows the GlcNAcTII (upper plot) and GalT (lower plot) activities induced by the vectors encoding these enzymes in orientations A and B. Column B shows the ST6GalI (upper plot) and ST3GalIII (lower plot) activities induced by the vectors encoding these enzymes in orientations A and B. Column C shows the sialic acid (upper plot) and CMP-sialic acid (lower plot) levels induced by the vectors encoding these enzymes in orientations A and B. The background levels in each assay were determined using extracts of mock-transfected Sf9 cells and are shown by the bars labeled "None". SfSWT3 and CHO refer to extracts of a transgenic insect cell line [15, 16] or Chinese hamster ovary cells, which served as positive controls for these assays.

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BMC Biotechnology

ISSN: 1472-6750

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