Figure 7
Quantitative GUS activity in transgenic tomato vegetative and floral organs. GUS activity was quantified in whole expanding leaves, whole flowers, and mature roots using 4-methylumbelliferone [MU] as substrate. Protein concentration was determined by the Bradford assay (Bradford 1976). Measurements were made on two reps of each independent transgenic line and an untransformed control. Activity in the untransformed control was subtracted from each transgenic sample. Ten independent B and C lines and seven independent A lines were analyzed. Each point represents a single transgenic line; the bars show the average of GUS activities for all transgenic lines of each construct.