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Figure 3 | BMC Biotechnology

Figure 3

From: Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models

Figure 3

A, Western blotting of the miniantibody MB-MoG-2.8 with reducing agents (lane 2), treated with glycosidase PNGase F (lane 1), and in not reducing not denaturing conditions (lane 3). B, Immunohistochemistry performed on histological section of mouse muscle tissue with the miniantibodies constructs MB-MoG-2.8 and 3.7. Secondary antibodies: biotinylated mAb SV5 followed by streptavidin conjugated with alkaline phosphatase (western blotting) or horseradish peroxidase (immunohistochemistry).

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