Figure 2
From: Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models
ELISA of supernatants of cultured HEK 293T cells transfected with the series of plasmids pMB-SV5 carrying 2.8 scFv gene (A and B) and 3.7 scFv gene (C and D) fused to CH2-CH3 domains genes from human, mouse and rat. Antigens: human tTG, mouse tTG and BSA. Secondary antibodies: A) and C) biotinylated mAb SV5 and streptavidin conjugated with peroxidase; B) and D) goat anti human, mouse and rat IgG or IgA conjugated with peroxidase.