Figure 1From: Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteinsThe primer design strategy using two pairs of primers. Primer F2 and R2 contained attB sites and no gene specific region, which could be synthesized in bulk; Primer F1 and R1 contained gene specific sequences and an overlap region with Primer F2 and R2. CDS stands for coding sequence and a protease cleavage site was engineered after attB1 site.Back to article page