Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor

Table 1 K_M and V_max values of AOX towards methanol for natural and mutant H. polymorpha strains.

H. polymorpha strain	К_М, mM	V_max, μmol of atomic O·min^-1·mg^-1 of protein at 20°C	Sigma factor, σ	Linear regression coefficient R for reciprocal plot
DL-1-356	0.62	27.4	0.084	0.992
CA2	2.48	66.7	0.040	0.958*
CA4	1.10	31.3	0.130	0.990

* A relatively low value of linear regression coefficient R for reciprocal plot for CA2-AOX can be explained by decreased activity measured and with accordingly increased error value

ISSN: 1472-6750