Figure 2

Agarose gel of PCR products from the Drosophila pseudolibrary. 5 μl samples of the PCR reactions were run on a 1.2% Agarose gel in 1 × TBE for 30 min. The DNA-bands were scored as indicated. Lanes: 1–8 Dicer 1 RNAse III; 9–16 Dicer 2 RNAse III; 1,13 Herculase 100 nM primers; 2,9,14 Phusion HF buffer, 100 nM primers; 3,15 Phusion HF buffer, 1 μM primers; 4,16 Phusion GC-TMSO buffer, 1 μM primers; 5 Pfu/Taq 1 μM primers; 6 Pfu/Taq-TMSO 1 μM primers; 7 Herculase 1 μM primers; 8 Herculase-TMSO 1 μM; 10 Phusion GC-TMSO buffer, 100 nM primers; 11 Pfu/Taq 100 nM primers; 12 Pfu/Taq-TMSO 100 nM primers. HF- and GC-buffers are intended to favor high-fidelity PCR of templates with low or high GC-content, respectively. Markers: 100 bp DNA ladder Plus (Fermentas), top band 3000 bp, strong band 500 bp.