Skip to main content

Table 1 Hydrogen uptake activity with various E. coli BW25113 mutants in complex medium as determined by the oxidized methylviologen-based H2 uptake assay after 5 min

From: Inhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystissp. PCC 6803

Strain Description Hydrogen uptake
   nmol/min/mg proteina relative
E. coli BW25113/pBS(Kan) wild type 94 ± 12 3.3
E. coli BW25113/pBS(Kan)Synhox wild type + HoxEFUYH 29 ± 7 1
E. coli BW25113 hyaA/pBS(Kan)Synhox ΔhyaA (defective hydrogenase 1) + HoxEFUYH 59 ± 21 2.0
E. coli BW25113 hyaB/pBS(Kan)Synhox ΔhyaB (defective hydrogenase 1) + HoxEFUYH 59 ± 6 2.0
E. coli BW25113 hybB/pBS(Kan)Synhox ΔhybB (defective hydrogenase 2) + HoxEFUYH 73 ± 13 2.5
E. coli BW25113 hybC/pBS(Kan)Synhox ΔhybC (defective hydrogenase 2) + HoxEFUYH 80 ± 11 2.8
E. coli BW25113 hycE/pBS(Kan) ΔhycE (defective hydrogenase 3) 25 ± 0.8 0.9
E. coli BW25113 hycE/pBS(Kan)Synhox ΔhycE (defective hydrogenase 3) + HoxEFUYH 7 ± 2 0.3
E. coli BW25113 hycG/pBS(Kan)Synhox ΔhycG (defective hydrogenase 3) + HoxEFUYH 10 ± 2 0.4
E. coli BW25113 hyaB hybC/pBS(Kan) ΔhyaB and ΔhybC (defective hydrogenase 1 and 2) 21 ± 1 0.7
E. coli BW25113 hyaB hybC/pBS(Kan)Synhox ΔhyaB and ΔhybC (defective hydrogenase 1 and 2) + HoxEFUYH 21.6 ± 0.3 0.8
E. coli BW25113 hyaB hybC hycE/pBS(Kan) ΔhyaB, ΔhybC, and ΔhycE (defective hydrogenase 1, 2, and 3) 1.8 ± 0.4 0.06
E. coli MC4100/pBS(Kan) wild type 35 ± 15 2.3
E. coli MC4100/pBS(Kan)Synhox wild type + HoxEFUYH 15 ± 6 1
E. coli TG1/pBS(Kan) wild type 16 ± 7 3.3
E. coli TG1/pBS(Kan)Synhox wild type + HoxEFUYH 5 ± 3 1
E. coli HD705/pBS(Kan) defective hydrogenase 3 8 ± 1 5.4
E. coli HD705/pBS(Kan)Synhox defective hydrogenase 3 + HoxEFUYH 2 ± 2 1
  1. Standard deviations shown from one representative experiment with 2 replicates. a IPTG was added for hydrogen uptake (1 mM) assays activity for 6 h in complex medium.