Table 1 Hydrogen uptake activity with various E. coli BW25113 mutants in complex medium as determined by the oxidized methylviologen-based H2 uptake assay after 5 min
Strain | Description | Hydrogen uptake | |
|---|---|---|---|
nmol/min/mg proteina | relative | ||
E. coli BW25113/pBS(Kan) | wild type | 94 ± 12 | 3.3 |
E. coli BW25113/pBS(Kan)Synhox | wild type + HoxEFUYH | 29 ± 7 | 1 |
E. coli BW25113 hyaA/pBS(Kan)Synhox | ΔhyaA (defective hydrogenase 1) + HoxEFUYH | 59 ± 21 | 2.0 |
E. coli BW25113 hyaB/pBS(Kan)Synhox | ΔhyaB (defective hydrogenase 1) + HoxEFUYH | 59 ± 6 | 2.0 |
E. coli BW25113 hybB/pBS(Kan)Synhox | ΔhybB (defective hydrogenase 2) + HoxEFUYH | 73 ± 13 | 2.5 |
E. coli BW25113 hybC/pBS(Kan)Synhox | ΔhybC (defective hydrogenase 2) + HoxEFUYH | 80 ± 11 | 2.8 |
E. coli BW25113 hycE/pBS(Kan) | ΔhycE (defective hydrogenase 3) | 25 ± 0.8 | 0.9 |
E. coli BW25113 hycE/pBS(Kan)Synhox | ΔhycE (defective hydrogenase 3) + HoxEFUYH | 7 ± 2 | 0.3 |
E. coli BW25113 hycG/pBS(Kan)Synhox | ΔhycG (defective hydrogenase 3) + HoxEFUYH | 10 ± 2 | 0.4 |
E. coli BW25113 hyaB hybC/pBS(Kan) | ΔhyaB and ΔhybC (defective hydrogenase 1 and 2) | 21 ± 1 | 0.7 |
E. coli BW25113 hyaB hybC/pBS(Kan)Synhox | ΔhyaB and ΔhybC (defective hydrogenase 1 and 2) + HoxEFUYH | 21.6 ± 0.3 | 0.8 |
E. coli BW25113 hyaB hybC hycE/pBS(Kan) | ΔhyaB, ΔhybC, and ΔhycE (defective hydrogenase 1, 2, and 3) | 1.8 ± 0.4 | 0.06 |
E. coli MC4100/pBS(Kan) | wild type | 35 ± 15 | 2.3 |
E. coli MC4100/pBS(Kan)Synhox | wild type + HoxEFUYH | 15 ± 6 | 1 |
E. coli TG1/pBS(Kan) | wild type | 16 ± 7 | 3.3 |
E. coli TG1/pBS(Kan)Synhox | wild type + HoxEFUYH | 5 ± 3 | 1 |
E. coli HD705/pBS(Kan) | defective hydrogenase 3 | 8 ± 1 | 5.4 |
E. coli HD705/pBS(Kan)Synhox | defective hydrogenase 3 + HoxEFUYH | 2 ± 2 | 1 |