In vitro recombination of allele replacement vectors into DEST-BAC, transformation, and visualization of resultant phenotypes. (A) Schematic diagram of allele replacement vectors featuring DsRed2, A33R, and B5R. (B) Four visual phenotypes of colony transformants observed after in vitro recombination between DEST-BAC and pDsRed2 and transformation. Colony transformants from a typical transformation were replated onto a chloramphenicol-containing agar plate to demonstrate phenotype differences. Numbers to the right of the plate refer to the actual numbers of each colony phenotype observed on the original transformation plate.