Figure 7
From: Engineering proteinase K using machine learning and synthetic genes
Changes in activity and half-life in designed protein variants. Activity (unheated) and half life were calculated for 13 protein variants and wild type proteinase K. The activity without heating was calculated from the initial slopes of the A405 measurements without heating (white bars), examples shown in Figure 6. The half-life at 68°C (shaded bars) was calculated using the initial slopes after different heating times and fitting to an exponential curve. Error bars represent one standard deviation of the experimental measurements. The wild-type values are shown on the left. The substitutions of each variant are given in the column below the variant name. Only 10 of the 19 positions are shown. In the remaining 9 positions, all variants contained amino acids from the wild-type sequence.