Figure 2
From: Engineering proteinase K using machine learning and synthetic genes
Three cycles of proteinase K variant design and testing. Mean activity measurements of the 3 sets of proteinase K variants are shown. Set 1 (diamonds) is the initial set of 59 variants. Set 2 (squares, 20 variants) was designed using the activities of Set 1. Set 3 (triangles, 16 variants) was designed based on sets 1 and 2. Activities towards N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide were measured at 37°C following a 5 minutes heat treatment of the enzyme at 68°C. Activities are expressed relative to the mean activity of 2 replicates of the wild-type proteinase K.