Table 1 DNA transformation efficiency of L. lactis spp. lactis cells with and without pretreatment with LiAc and DTT. Results are the mean (± SD) of 6 replications
LiAc (mM) | DTT (mM) | Transformants/μg DNA | |
|---|---|---|---|
L. lactis LM0230 | L. lactis ATCC 11454 | ||
0 | 0 | 1.20 ± 0.50 × 105 | 1.00 ± 0.48 × 104 |
100 | 0 | 1.10 ± 0.52 × 105 | 1.20 ± 0.50 × 104 |
0 | 10 | 13.00 ± 8.02 × 106 | 7.00 ± 5.01 × 105 |
100 | 10 | 225.00 ± 52.51 × 107 | 117.00 ± 42.55 × 106 |