Figure 10
Antigen binding ELISA of soluble antibody fragments produced in P. pastoris with 100 ng/well lysozyme or BSA coated per well. A serial dilution of gel filtration purified fractions of scFabΔC ranging from 8 ng/mL to 1 μg/mL were applied, the D1.3 antibody fragments were detected using Protein L HRP conjugate (1:10000). Measurements were done in duplicate.