Figure 1

Vectors constructed for this study. A illustration of the different antibody formats, B the pHAL1-D1.3 vectors, C linker sequences of the scFab variants. Abbreviations: lacZ promoter: promoter of the bacterial lac operon; RBS: ribosome binding site; pelB: signal peptide sequence of bacterial pectate lyase, mediating secretion into the periplasmic space; VH: variable fragment of the heavy chain; LC: light chain; ochre: ochre stop codon; amber: amber stop codon; strep-tag II: synthetic tag binding to streptactin. The elements of the inserts are not drawn to scale.