Figure 3
From: Criteria for effective design, construction, and gene knockdown by shRNA vectors
DNA sequencing of pHSPG-shTLR4 using modified reaction conditions. DNA sequencing peaks are shown in a full scale view where base positions are indicated by the row of numbers in each panel and the Y axis is the signal intensity. Sequencing reaction conditions shown are BigDye v1.1 (BD) chemistry (A), 0.83 M Betaine + 1 ×PCRx Enhancer in BD chemistry (B), 10:1 BD:dGTP chemistries (C), 0.83 M Betaine + 1 ×PCRx Enhancer in 10:1 BD:dGTP chemistries (D), and 1 × ThermoFidelase I in 10:1 BD:dGTP chemistries (E). The drop in signal (step in peak height) at the hairpin is highlighted by an arrow in the 10:1 BD:dGTP chemistries panel.