Figure 2
From: Criteria for effective design, construction, and gene knockdown by shRNA vectors
Gene expression analysis for wild-type and mutant shRNA vectors prepared simultaneously using wild-type/mutant double stranded hybrids. (A) Sequences of the target sites for four wild-type and mutant shRNA vectors that were prepared simultaneously as detailed in Figure 1. (B) Realtime analysis of shRNA knockdown, and loss of knockdown by mutant shRNA vectors from (A). Values are standardized to 100% in non-transduced THP1 cells. The expression in THP1 cells transduced with an empty vector (EV) is shown as an additional control. Values represent average +SEM for at least three assays performed in duplicate.