Figure 9

Fluorescence and confocal microscope analysis of VLP challenged cells. (A) Fluorescence microscope analysis of CEMss cells 4 hours after the challenge with VSV-G (on the left) or X4 HIV-1 Env (on the right) pseudotyped NefG3C-GFP VLPs after DAPI labeling. The bars mark 10 μM (B) Confocal microscope analysis of CEMss cells 4 hours after the challenge with (VSV-G) NefG3C-GFP VLPs. The same field was scanned at three section plans. The images are the result of the computer-assisted analysis carried out by overlapping the images obtained with visible and fluorescence lights. The bars mark 10 μM.