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Table 1 Castillo et al., Table 1 Kinetic parameters of subtilisin C. co-lyophilized with methyl-β-cyclodextrin

From: On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg

 

1,4-Dioxane

 

Day 0

Day 4

Vmaxa

102 ± 3

16 ± 2

KMb

130 ± 10

319 ± 48

Vmax/KM

0.78

0.050

  1. amMmin-1, bmM, both values were obtained plotting the initial velocity (V) vs (V/[S]). Model reaction: transesterification between phenyl alcohol and vinyl butyrate at 45°C.
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BMC Biotechnology

ISSN: 1472-6750

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