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Table 1 Castillo et al., Table 1 Kinetic parameters of subtilisin C. co-lyophilized with methyl-β-cyclodextrin

From: On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg

  1,4-Dioxane
  Day 0 Day 4
Vmaxa 102 ± 3 16 ± 2
KMb 130 ± 10 319 ± 48
Vmax/KM 0.78 0.050
  1. amMmin-1, bmM, both values were obtained plotting the initial velocity (V) vs (V/[S]). Model reaction: transesterification between phenyl alcohol and vinyl butyrate at 45°C.