Figure 2

p3-siRNA is able to produce three different siRNAs to simultaneously suppress the expression of three different genes. a) Construction of TR126 and three control vectors: TR1, TR2 and TR3. Sequence of Si1, Si2 and Si6 are shown in additional files (Additional file 2). b) Schematic structure of retroviral vector pMX-siRNA. c) – e) Western analysis showing that TR126 is able to suppress the expression of TRAF1 c); TRAF2 d) and TRAF6 e). C: uninfected BMMs controls. Actin was probed as loading control. The expression levels of TRAF proteins were normalized to that of actin. These assays were independently repeated three times and a representative data is shown.