Figure 2

Activity of bifunctional oligonucleotides containing a 3' tail. The Bcl-x pre-mRNA was incubated in HeLa extracts in the presence of increasing amounts of oligonucleotides (panel A, BS indicating the presence of a branch site sequence in the tail). Each mixture was tested in triplicate. (B) RT-PCR analysis of splicing mixtures. The ratio of the Bcl-xL and Bcl-xS products was plotted for different concentrations of oligomers. (C) Profiles obtained on the Agilent 2100 bioanalyzer are shown for a control mixture (lane 2) and mixtures incubated with the M4, M4B5 and M4B3+ oligonucleotides at concentrations of 18.75, 37.5 and 75 nM.