Figure 5
From: Multiplexed expression and screening for recombinant protein production in mammalian cells
Determination of dot blot denaturation conditions to use. Purified EfnB2-EC in mock supernatant was incubated in urea at different concentrations for 1 hour at room temperature prior to loading onto a nitrocellulose membrane in triplicate using the dot blot apparatus. After detection of His tagged proteins (see Methods section) with anti-His tag mAb and Cy5 labelled goat anti-mouse antibody the blots were scanned on a Typhoon 8600 variable mode imager (A) and dots were quantitated using ImageQuant software (B).