Table 1 A summary of the pSANG expression vector series, their scFv properties, and E. coli strains used.
From: A simple vector system to improve performance and utilisation of recombinant antibodies
Vector Name | pSANG10-3F | pSANG11-3F | pSANG12 | pSANG13 | pSANG14-3F | pSANG15-3F | pSANG16 | pSANG17 | pSANG18-3F |
|---|---|---|---|---|---|---|---|---|---|
Valency | Monovalent | Bivalent | Monovalent | Bivalent | Bivalent | Monovalent | Monovalent | Bivalent | Monovalent |
Epitope Tag(s) | 6xHis Tri-Flag | 6xHis Tri-Flag | 6xHis | 6xHis | 6xHis Tri-Flag | 6xHis Tri-Flag | 6xHis | 6xHis | 6xHis Tri-Flag |
Site of scFv accumulation | Periplasm | Periplasm | Periplasm | Periplasm | Periplasm | Cytoplasm | Cytoplasm | Cytoplasm | Cytoplasm |
Additional modifications | None | C-terminal Cysteine | MBP | MBP, C-terminal Cysteine | Bacterial Alkaline Phosphatase | Leaderless | Leaderless, MBP | Leaderless, MBP, C-terminal Cysteine | Leaderless, C-terminal Cysteine |
Expression Host tested | BL21(DE3) Origami 2 | BL21(DE3) | BL21(DE3) | BL21(DE3) | BL21(DE3) Origami 2 | Origami 2 | Origami 2 | Origami 2 | Origami 2 |
Protein expressed | scFv | scFv-cys | scFv-MBP | scFv-MBP-cys | scFv-AP | scFv | scFv-MBP | scFv-MBP-cys | scFv-cys |