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Table 1 A summary of the pSANG expression vector series, their scFv properties, and E. coli strains used.

From: A simple vector system to improve performance and utilisation of recombinant antibodies

Vector Name pSANG10-3F pSANG11-3F pSANG12 pSANG13 pSANG14-3F pSANG15-3F pSANG16 pSANG17 pSANG18-3F
Valency Monovalent Bivalent Monovalent Bivalent Bivalent Monovalent Monovalent Bivalent Monovalent
Epitope Tag(s) 6xHis Tri-Flag 6xHis Tri-Flag 6xHis 6xHis 6xHis Tri-Flag 6xHis Tri-Flag 6xHis 6xHis 6xHis Tri-Flag
Site of scFv accumulation Periplasm Periplasm Periplasm Periplasm Periplasm Cytoplasm Cytoplasm Cytoplasm Cytoplasm
Additional modifications None C-terminal Cysteine MBP MBP, C-terminal Cysteine Bacterial Alkaline Phosphatase Leaderless Leaderless, MBP Leaderless, MBP, C-terminal Cysteine Leaderless, C-terminal Cysteine
Expression Host tested BL21(DE3) Origami 2 BL21(DE3) BL21(DE3) BL21(DE3) BL21(DE3) Origami 2 Origami 2 Origami 2 Origami 2 Origami 2
Protein expressed scFv scFv-cys scFv-MBP scFv-MBP-cys scFv-AP scFv scFv-MBP scFv-MBP-cys scFv-cys