Figure 7
From: A simple vector system to improve performance and utilisation of recombinant antibodies
Effect of alkaline phosphatase fusion on performance in Western blot. scFv-APs demonstrate improved sensitivity in Western blot. Recombinant human desmin was detected with equimolar concentrations of anti-desmin scFv clone C10 (applied at a concentration of 2 × 10-8 M) expressed as either scFv or scFv-AP formats. Detection was by anti-FLAG antibody coupled to horse-radish peroxidase and addition of ECL western-blotting substrate.