Figure 2

Effects of hazel and yew cell culture extracts on mitosis. DNA was visualised with DAPI (blue), mitotic spindles with a β-tubulin antibody (red) and centrosomal structures with a γ-tubulin antibody (green-yellow). Mock treated cells (A-D); 10 nM Taxol treated cells (E); hazel medium extract treated cells (F); and yew medium extract treated cells (G). Normal metaphases (A, B); anaphases (C); and telophases (D) were mainly found in mock treated cells. Multipolar metaphases (E, F) and multipolar metaphases or anaphases with displaced chromosomes (F, G arrows) were mainly found in treated cells.