Figure 4

Fluorescence ratios generated by Two-Temperature LATE-PCR genotyping are independent of starting genome copy number. This figure shows a plot of the ratio of Cy5 readings at 50°C and 25°C derived from a LATE-PCR amplification of the genomic DNA segment containing the rs858521 [C/G] SNP monitored with a ResonSense^® probe against the rs858521 C allele. Red lines, homozygous CC replicates containing 2000 starting genomes; red dotted lines, homozygous CC replicates containing 100 starting genomes. Both sets of replicate homozygous samples generate the same two-temperature fluorescence ratio even though these samples differ in their amount of starting material.