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Figure 7 | BMC Biotechnology

Figure 7

From: Flip-Flop HSV-BAC: bacterial artificial chromosome based system for rapid generation of recombinant herpes simplex virus vectors using two independent site-specific recombinases

Figure 7

Phenotype of recombinant viruses generated by the Flip-Flop HSV-BAC system. (a) Time course of ICP4 expression in Vero cells infected with recombinant viruses. bM24-CMV induced weak expression of ICP4 as early as 4 hours post-infection (pi), which increased over time up to 24 hours pi. Vero cells infected with bM24-null and bM24-empty showed no detectable ICP4 expression. hrR3 and KOS which retain two copies of the wild-type ICP4 gene showed very strong expression of ICP4 after 8 hours pi. (b) Plaque forming assay on Vero cells. Vero cells grown in 6-well dishes were infected with the indicated amounts of recombinant virus, fixed five days after infection and stained with X-gal. A few tiny plaques are visible in the bM24-null well.

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BMC Biotechnology

ISSN: 1472-6750

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